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polyclonal sheep anti bovine igg  (Bio-Rad)


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    Bio-Rad polyclonal sheep anti bovine igg
    Polyclonal Sheep Anti Bovine Igg, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal sheep anti bovine igg/product/Bio-Rad
    Average 93 stars, based on 76 article reviews
    polyclonal sheep anti bovine igg - by Bioz Stars, 2026-02
    93/100 stars

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    Immunization with DNA vaccines encoding for bp26 and trigger factor (TF) elicits serum immunoglobulin G (IgG) antibody responses. Bison (four/group) were immunized intramuscularly with pcDNA3.1 vector (600 μg) or a combination of pCMVbp26 (300 μg) and pCMVTF (300 μg), together with 50 μg CpG as adjuvant on days 0, 14, and 28. Serum IgG anti-bp26 and anti-TF titers were measured by enzyme-linked immunosorbent assay on wk 8, 10, and 12 after primary immunization. The IgG, IgG1 and <t>IgG2</t> (A) anti-bp26 and (B) anti-TF endpoint titers peaked between wk 8 and 12 after primary immunization. Control bison immunized with control vector, pcDNA3.1 plus CpG failed to elicit antibodies to bp26 and TF. Asterisks represent significant differences in IgG responses versus pcDNA3.1 vector-immunized bison (P≤0.05).
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    Immunization with DNA vaccines encoding for bp26 and trigger factor (TF) elicits serum immunoglobulin G (IgG) antibody responses. Bison (four/group) were immunized intramuscularly with pcDNA3.1 vector (600 μg) or a combination of pCMVbp26 (300 μg) and pCMVTF (300 μg), together with 50 μg CpG as adjuvant on days 0, 14, and 28. Serum IgG anti-bp26 and anti-TF titers were measured by enzyme-linked immunosorbent assay on wk 8, 10, and 12 after primary immunization. The IgG, IgG1 and <t>IgG2</t> (A) anti-bp26 and (B) anti-TF endpoint titers peaked between wk 8 and 12 after primary immunization. Control bison immunized with control vector, pcDNA3.1 plus CpG failed to elicit antibodies to bp26 and TF. Asterisks represent significant differences in IgG responses versus pcDNA3.1 vector-immunized bison (P≤0.05).
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    Immunization with DNA vaccines encoding for bp26 and trigger factor (TF) elicits serum immunoglobulin G (IgG) antibody responses. Bison (four/group) were immunized intramuscularly with pcDNA3.1 vector (600 μg) or a combination of pCMVbp26 (300 μg) and pCMVTF (300 μg), together with 50 μg CpG as adjuvant on days 0, 14, and 28. Serum IgG anti-bp26 and anti-TF titers were measured by enzyme-linked immunosorbent assay on wk 8, 10, and 12 after primary immunization. The IgG, IgG1 and IgG2 (A) anti-bp26 and (B) anti-TF endpoint titers peaked between wk 8 and 12 after primary immunization. Control bison immunized with control vector, pcDNA3.1 plus CpG failed to elicit antibodies to bp26 and TF. Asterisks represent significant differences in IgG responses versus pcDNA3.1 vector-immunized bison (P≤0.05).

    Journal: Journal of wildlife diseases

    Article Title: DNA VACCINATION OF BISON TO BRUCELLAR ANTIGENS ELICITS ELEVATED ANTIBODY AND IFN-γ RESPONSES

    doi: 10.7589/0090-3558-47.3.501

    Figure Lengend Snippet: Immunization with DNA vaccines encoding for bp26 and trigger factor (TF) elicits serum immunoglobulin G (IgG) antibody responses. Bison (four/group) were immunized intramuscularly with pcDNA3.1 vector (600 μg) or a combination of pCMVbp26 (300 μg) and pCMVTF (300 μg), together with 50 μg CpG as adjuvant on days 0, 14, and 28. Serum IgG anti-bp26 and anti-TF titers were measured by enzyme-linked immunosorbent assay on wk 8, 10, and 12 after primary immunization. The IgG, IgG1 and IgG2 (A) anti-bp26 and (B) anti-TF endpoint titers peaked between wk 8 and 12 after primary immunization. Control bison immunized with control vector, pcDNA3.1 plus CpG failed to elicit antibodies to bp26 and TF. Asterisks represent significant differences in IgG responses versus pcDNA3.1 vector-immunized bison (P≤0.05).

    Article Snippet: Horseradish peroxidase–conjugated mouse anti-bovine IgG monoclonal antibody (mAb; clone IL-AR; Serotec, Raleigh, North Carolina, USA) and sheep anti-bovine IgG1 and IgG2 polyclonal antibodies (Novus Biologicals, Littleton, Colorado, USA) were used for detection.

    Techniques: Vaccines, Plasmid Preparation, Adjuvant, Enzyme-linked Immunosorbent Assay, Control